Cell Function Reagents and Kits
Aat Bioquest Calcein AM CAS 148504-34-1 (1 mg)
Calcein AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity as compared to calcein. This catalog is for 1 mg.
Aat Bioquest Fura-8 AM (1 mg)
Although Fura-2 has become the preferred excitation-ratioable calcium indicator of choice it has certain limitations e.g. lower sensitivity than single wavelength calcium indicators such as Fluo-8 and Cal-520. This catalog is for 1 mg.
Apexbio Technology LLC CFDA-SE(Synonyms: CFSE, Carboxyfluorescein succinimidyl ester, 5-(and-6)-Carboxyfluorescein diacetate succinimidyl ester), 10mg, CAS: 150347-59-4.
CFDA-SE (Carboxyfluorescein diacetate succinimidyl ester CAS 150347-59-4) is a membrane-permeant fluorescent dye commonly utilized for labeling viable cells and monitoring cellular proliferation Upon entering cells intracellular esterases hydrolyze CFDA-SE into carboxyfluorescein succinimidyl ester (CFSE) which becomes fluorescent (excitation 494 nm emission 521 nm) binds covalently to intracellular amino groups and is thereafter trapped within the cell cytoplasm Each cell division results in halving fluorescence intensity enabling accurate quantification of successive proliferation events via flow cytometry CFDA-SE labeling methodology has extensive applications in assessing lymphocyte fibroblast NK cell and bacterial proliferation both in vitro and in vivo
Aat Bioquest Calcein AM UltraPure grade CAS 148504-34-1 (1 mg)
Calcein AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity as compared to calcein. This catalog is for 1 mg.
Medchemexpress LLC HY-D0972 100mg Medchemexpress, Thioflavine S CAS:1326-12-1 Purity:>98%
Medchemexpress, HY-D0972 100mg Thioflavine S CAS:1326-12-1 Thioflavine S is a fluorescent histochemical marker of dense core senile plaques. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Cayman Chemical Calcein Red™ AM, Purity: ≥90%, 1 mg
A fluorogenic dye used to assess cell viability; ex/em = 560/574 nm, respectively
Biolegend Zombie R718™ Fixable Viability Kit Apps: FC; Size: 500 tests
Zombie R718™ Fixable Viability Kit Apps: FC; Size: 500 tests
Lonza Walkersville ToxiLight Non-Destructive Cytotoxicity BioAssay Kit, 1000 Test
The ToxiLight BioAssay Kit is a bioluminescent, non-destructive cytolysis assay kit designed to measure the release of the enzyme, adenylate kinase (AK), from damaged cells. AK is a robust protein present in all eukaryotic cells, which is released into the culture medium when cells die. The enzyme actively phosphorylates ADP to form ATP and the resultant ATP is then measured using the bioluminescent firefly luciferase reaction. As the level of cytolysis increases, the amount of AK in the supernatant also increases, which results in emission of higher light intensity by the ToxiLight reagent. Because the ToxiLight BioAssay Kit exploits the fact that AK is released from cells when they die, there is no need for cell lysis (unlike many other cytotoxicity assays). Repeated samples of supernatant can therefore be taken over time without disrupting the cells themselves. This allows for kinetic analysis of cell death.
TRIBIOSCIENCE INC XTT Cell Viability Assay, 500 Tests
Tribioscience’s XTT Cell Viability Assay Kit is a colorimetric assay to quantify the viable cells with soluble formazan dye. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds.
Medchemexpress LLC HY-D0984A 5mg Medchemexpress, TMRM Perchlorate CAS:115532-50-8 Purity:>98%
Medchemexpress, HY-D0984A 5mg TMRM Perchlorate CAS:115532-50-8 TMRM Perchlorate is a cell-permeant cationic lipophilic red fluorescent dye (λ ex =530 nm, λ em =592 nm). Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Abcam LysoLive™ Lysosomal Acid Lipase Assay Kit.
LysoLive™ Lysosomal Acid Lipase Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement
Biotium Resazurin, sodium salt
Resazurin is the N-oxide of the fluorescent dye resorufin and used to detect reductive activities in cells. It is widely used for measuring cell proliferation and mitochondrial metabolic activity. Resazurin itself is non-fluorescent until reduced to the highly red fluorescent resorufin (Ex/Em: 563/587 nm) in the presence of reductants like NADPH or NADH and the enzyme diaphorase. Resazurin can therefore be used to detect NADH, NADPH, or diaphorase levels. Furthermore, the resazurin /diaphorase /NADPH system can be used to detect any biochemical or enzyme activity involved in generating NADH or NADPH. Although resazurin is available from several suppliers, trace amounts of the resorufin contaminant often make the material unsuitable for bioassay applications. This high-grade resazurin has a minimal background fluorescence. Properties: Ex/Em (pH 9) = 604 nm/none; Ex/Em (reduced): 563/587 nm; Dark solid soluble in DMSO or water; Store at 4°C protected from light; C12H6NNaO4; MW: 251.17
Biotium Sulforhodamine 101
Sulforhodamine 101 has a longer absorption wavelength than does sulforhodamine B. Like sulforhodamine B and G, in addition to their potential use in cancer drug screening, these fluorescent dyes have been primarily used as polar tracers for the studies of neuronal cell morphology and cell-cell communication. Properties: Ex/Em = 586/605 nm; Red solid soluble in water; Store at room temperature; C31H30N2O7S2; MW: 607; [60311-02-6]
Biotium Fura-2, AM Ester, 1mM In Anhydrous DMSO
Fura-2, AM ester is an intracellular fluorescent calcium indicator, and the preferred dye for ratiometric imaging microscopy. The membrane-permeant AM ester can be loaded into cells via incubation, but due to limited water solubility, a mild detergent Pluronic F-127 is often used to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2, but intracellular esterases readily hydrolyze it to Fura-2. Upon calcium binding, the excitation maximum of Fura-2 undergoes a blue-shift from 363 nm (no Ca2) to 335 nm (high Ca2) while the emission maximum is relatively unchanged. Measure by flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C44H47N3O24; MW: 1001.9; [108964-32-5].